不同接苯时间苯中毒患者胸腺近期输出功能变化

The change of recent thymic output function among patients exposed to benzene for varied periods

为分析职业性苯中毒工人外周血单个核细胞中T细胞受体删除DNA环(TRECs)的含量,了解苯中毒工人胸腺近期输出功能变化,采用实时荧光定量聚合酶链反应(real time PCR)和TaqMan方法检测苯中毒和健康人外周血单个核细胞中TRECs的含量。结果显示,TRECs含量苯中毒观察对象2·97±1·31、轻度苯中毒1·73±1·11、中度苯中毒2·28±1·44、重度苯中毒1·91±1·95,各组患者每1000个外周血单个核细胞中TRECs含量均明显低于健康组7·39±6·83(P<0·05),轻度和重度苯中毒组TRECs含量显著低于苯中毒观察对象组(P<0·05)。重度苯中毒组接苯时间>3的工人TRECs含量显著低于接苯时间≤3年者,苯中毒观察对象组接苯时间>5年的工人TRECs含量显著低于≤5年者(P<0·05)。提示苯中毒患者胸腺近期输出功能低下。

To analyze the content of T cell receptor excision DNA circles （TRECs） within peripheral blood rnononuclear cells （PBMCs）, thereby to evaluate the recent thymic output function in patients exposed to benzene. Quantitative detection of TRECs in DNA of PBMCs was preformed by real time PCR using TaqMan technique in patients with benzene poisoning and normal individual. The result showed that the level of TRECs in dubitable benzene-poisoned 2.97 ±1.31, chronic mild benzene-poisoned 1.73 ± 1.11, chronic middling benzene-poisoned 2.28 ± 1.44 and chronic severely benzene-poisoned 1.91 ± 1.95 decreased significantly compared with that of in normal individuals （ P 〈 0.05）. The level of TRECs among patients for exposed to benzene more than 3 years in chronic severely benzene-poisoned was decreased significantly compared with that of among patients for exposed to benzene less than 3 years （ P 〈0.05）. The level of TRECs among patients for exposed to benzene more than 5 years in dubitable benzene-poisoned groups was decreased significantly compared with that of among patients for exposed to benzene less than 5 years （P 〈0.05）. The results indicated that the recent thymic output function was decreased in patients with benzene poisoning. Low dose exposure to benzene might cause cellular cellular disorder.