大鼠酒精性肝病模型的建立

趴的探讨白酒灌胃联合自由饮酒法建立大鼠早期酒精性肝病模型的意义,以便找到一种发病机制、病理改变均与临床接近且周期较短的方法来制作大鼠酒精性肝病模型。方法模型组采用52%二锅头白酒配置成40%、45%、50%浓度,分别灌胃4、4、2周,第11周起改用自由饮酒(浓度50%);对照组前10周用等容生理盐水灌胃,第11周起自由饮水。12周后测定大鼠血清ALT、AST、弊蛋白、白球比例,观察大鼠肝脏的组织学和超微结构改变。结果12周后,模型组大鼠血清AST较正常对照组明显增高,白蛋白水平、白球蛋白比例明显下降,肝脏组织有炎症、坏死和轻微脂肪变性、纤维化。结论白酒灌胃联合自由饮酒法是建立大鼠慢性酒精性肝病模型的一种可靠稳定方法,将为酒精性肝病的相关研究打下良好的实验室基础。

Objective To induce a relatively simple and feasible rat model of alcoholic liver disease by the freely drinking and perfusing stomach with alcohol, which is close clinical situation and timesaving . Methods Rat model of alcoholic liver disease was induced by the freely drinking and perfusing stomach with alcohol . The rats were intragastric administratered in first with a special amount of alcohol receiving alcohol drinking water as the sole source of liquid. The alcohol concentration was increased from 40% （ alcohol/water： v/v）to 45% and to a final concentration of 50% for 4,4,2 weeks by a tube everyday .Then the modal group drank alcohol freely from the llth week .In the control group, rats were intragastric administratered with equal NS in early 10 weeks,and drunk water freely from the llth week. After 12 weeks, almandine aminotransferase （ALT）, aspartateaminotransferase（AST）, albumin （ALB）, albumin/globulin（A/G） of rats drinking and perfusing stomach with alcohol were detected and were compared with those of the control rats. Used t - test for comparative method of two sample means. And the liver tissues were observed both in light microscope（LM） and electron microscope（EM）. Results After 12 weeks, AST of rats drinking and perfusing stomach with alcohol were increased, ALB and A/G were decreased, compared with those of the normal rats, and alcohol hepatitis were aggravated and fibrosis were observed in pathology and ultrastuctm＇e. Condusions These results demonstrate that the alcohol liver disease model we used is stable and can be used in study of ALD.