人结直肠癌组织中Smad^4蛋白和TGF-β及其Ⅱ型受体表达的临床意义

目的探讨Smad4蛋白和TGF-β及其Ⅱ型受体在人结直肠癌组织中表达的临床意义。方法应用免疫组织化学法,对76例结直肠癌组织标本进行检测,并与临床病理特点进行分析。结果Smad4蛋白在肿瘤组织和正常黏膜组织中的阳性率分别为35.5%和61.8%,差异有非常显著性(P<0.01);Smad4蛋白的表达与肿瘤分期和淋巴结转移相关(P<0.05);TGF-β蛋白在肿瘤组织和正常黏膜组织中的阳性率分别为25.0%和42.1%,差异有显著性(P<0.05);TGF-βⅡR蛋白在肿瘤组织和正常黏膜组织的阳性率分别为18.4%和21.1%,差异无显著性(P>0.05);Smad4、TGF-β、TGF-βⅡR与患者性别、年龄及肿瘤部位、大小、分化程度、有无瘤栓、组织类型、大体类型等差异均无显著性(P>0.05)。结论与正常黏膜组织相比,Smad4、TGF-β在肿瘤组织中表达下调,且Smad4低表达的结直肠癌大多分期较迟、淋巴结转移较多,而TGF-β表达与肿瘤分期及淋巴结转移无关;TGF-βⅡR在肿瘤组织与正常组织中表达差异无显著性,与肿瘤分期及淋巴结转移差异亦无显著性。

Objective To detect the expression of Smad4 protein, TGF - Ⅱ3 and TGF - β Ⅱ R protein in human colorectal cancer and analyze the relationship between these protein＇ s expression and clinical characteristics. Methods Total 76 patients pathological diagnosed with colorectal cancer by surgery were collected and the expression of Smad4 protein, TGF - βand TGF - β Ⅱ R protein were measured in both specimens of normal mucosa and tumor tissue with immunohistochemistry （ IHC ）. The relationship between the expression of Smad4 protein, TGF - βand TGF - β Ⅱ R protein and the clinic - pathological factors was analyzed. Resluts The positive rate of Smad4 protein expression in the tumor tissues and normal mucosa were 35.5 % （27/76） and 61.8% （47/76） respectively and their difference was significant statistically （ P 〈 0.01 ）. The positive rate of TGF - βprotein in tumor tissue and normal mucosa were 25.0 % （19/76） and 42.1% （32/76） respectively and their difference is significant statistically too （ P 〈 0.05 ）. The positive rate of TGF - β Ⅱ R protein in tumor and normal mucosa were 18.4% （14/76） and 21.1% （16/76） respectively, there was no significant difference statistically （P 〉 0. 05 ） ; The high level expression of Smad4 protein in tumor tissue was relative to the earlier tumor stage and fewer lymph node metastasis（ P 〈 0.05 ）. There was no relationship between Smad4 protein and gender, age, tumor location, tumor size, differentiation, tumor embolus, pathologic type, as well as TGF- β Ⅱ R. TGF- βprotein （ P 〉 0.05 ）. Conclusion Smad4 and TGF - βprotein are down - expressed in tumor tissue in contrast to normal mucosa in colorectal cancer patients and Smad4 protein＇s lower expression is always accompanied with later stage, more lymph node metastasis, But there is no significant difference between TGF- βxpression and tumor stage, lymph node metastasis. There is no significant difference of TGF- β Ⅱ R protein expressed in tumor tissue and normal mucosa.