摘要
以6年生“赤霞珠”葡萄的休眠芽为材料,应用高效液相色谱法测定葡萄休眠芽中的脱落酸(ABA)含量。葡萄休眠芽中的脱落酸用80%冷甲醇抽提,抽提液在石油醚和水相之间分配,后经过吡咯烷酮(PVP)净化和乙酸乙酯萃取,最后用甲醇、水作为流动相,在ODS-C18柱上进行分离,用紫外检测器在260nm波长下检测、鉴定。结果表明,高效液相色谱法检测赤霞珠葡萄休眠芽中内脱落酸,含量为2.177μg.g-1。
HPLC was evaluated for the determination of abscisic acid （ABA） in grapevine dormant buds. Firstly,the phytohormone in six- year- old grapevine dormant buds was extracted with methanol -water （80：20）, and then the sample extract was partitioned between ligroin and water. Secondly, ABA was purified further with insoluble polyvinyl pyrrolidone （PVP）. Thirdly, ABA was separated and quantified by C18 reversed phase HPLC with methanol- water at a flow rate of 0. 9ml. Finally, detection was made by a UV detector at 260nm. The result manifested that phytohormone in grapevine dormant buds was 2. 177μg·g^-1.
出处
《落叶果树》
2007年第3期4-6,共3页
Deciduous Fruits
