缺氧小鼠肾组织结构的变化

The changes of nephridial tissue structure in hypoxia mice

目的:通过观测缺氧后小鼠肾组织形态结构、碱性磷酸酶的量的变化,探讨不同缺氧状态下对肾脏结构和功能的影响。方法:用小鼠制作间歇性重复缺氧模型后,在不同时间点取小鼠肾组织分别作HE染色、过碘酸雪夫氏反应(PAS染色)和碱性磷酸酶染色法操作。结果:缺氧即时处理组肾实质内毛细血管扩张充血,碱性磷酸酶含量增加。但在缺氧延时处理组小鼠由于近曲小管受到损伤,其肾组织切片碱性磷酸酶染色变浅淡。该组近曲小管上皮细胞肿胀,出现颗粒变性。肾小体系膜区增宽,系膜细胞及基质增生。肾实质及肾间质血管扩张充血,间质水肿,中性粒细胞浸润。结论:缺氧时可导致小鼠近曲小管上皮细胞碱性磷酸酶含量增加,但其碱性磷酸酶染色强度随着缺氧时间的延长、近曲小管损伤的加重而成反比变化。

Objective： To investigate the effects on mouse nephridial tissue structure and function in different anoxic condition through observing the changes of nephridial tissue morphology and alkaline phosphatase （AKP） content in hypoxia mice. Methods ： we have constructed the intermittent hypoxia mouse model from which the nephridial tissue were taken in different hypoxia time points and were done HE staining, PAS reaction and AKP staining. Results： In the hypoxia mouse group immediately killed, blood capillary is ectatic and hyperaemic in renal parenchyma and the AKP content are increased. In the hypoxia mouse group killed later, the AKP staining intensity in nephridial tissue section is lighter than that in the control group which might be caused by impaired proximal convoluted tubules. In the later killed group, proximal convoluted tubule epithelial cellular swelling and granular degeneration appear, corpuscula renis mesangial region is widened, intercapillary cells and matrix are hyperplasia. There are angiectasia and hyperaemia, interstitial edema and neutrophil infiltrate in renal parenchyma and renal interstitium. Conclusion： This study shows that the AKP content is increased in mouse proximal convoluted tubule epithelial cell induced by hypoxia. However, the staining intensity of AKP is inverse ratio with the hypoxia time and proximal convoluted tubule damage.