固定化假丝酵母99-125脂肪酶催化合成甘油二酯

Synthesis of diglycerides by enzymatic esterification using immobilized lipase from Candida sp.99-125 in a solvent-free system

对无溶剂体系中自制固定化假丝酵母99-125脂肪酶催化油酸、甘油合成甘油二酯过程进行了研究,比较了自制固定化酶与商业酶在催化该反应上的不同,发现自然挥发脱水比真空脱水更适合自制固定化酶催化合成甘油二酯。对反应条件的研究表明:底物摩尔比2∶1,反应温度50℃,酶量0.6g/5.65g,自然挥发脱水条件下油酸酯化率可达92.64%,甘油二酯含量可达66.16%。

Enzymatic synthesis of diolein using oleic acid and glycerol as substrates for immobilized lipase from Candida sp. 99-125 has been studied in a solvent-free system. The immobilized lipase from Candida sp. 99- 125 and different commercial lipases were compared under same reaction conditions. Of three ways of removing water produced in the reaction, spontaneous evaporation was found to be most effective. After optimization of reaction conditions, the conversion of oleic acid can reach a maximum of 92.64 % and the amount of diacylglycerol （DAG） in the product can reach 66.16% when using a substrate ratio （oleic acid to glycerol） of 2： 1, reaction temperature of 50 ℃, and enzyme dosage of 0.60 g/5.65 g oleic acid.