中医不同治法对成纤维细胞生长因子在大鼠肝癌中表达的影响

Influence of Different Therapeutic Methods in TCM on Expression and Regulation of Fibroblast Growth Factor in Rats with Liver Cancer

目的:观察成纤维细胞生长因子(FGF)在二乙基亚硝胺(DEN)诱发大鼠肝癌组织中的表达及不同中医治法的作用。方法:采用DEN诱发大鼠肝癌,随机分为正常组、模型组、喃氟啶组、抑癌扶正行气活血法组、清热解毒法组、行气活血法组、健脾扶正法组,应用Affymetrix Rat230A GeneChip及相关技术检测各组肝脏(含肝癌)FGF基因表达的差异。结果:Rat230A芯片中有24个FGFs、3个FGFRs、1个FGFBP,其中肝癌表达增加者15个,减少者13个。FGF13芯片读数(肝癌形成/正常肝脏)为10.8,FGF7芯片读数(正常肝脏/肝癌形成)为13.1。近10年有关FGFs文献857篇,涉及肝癌者仅49篇,仅3个基因(FGF1、FGF2及FGFR3)涉及。结论:FGFs基因在大鼠肝癌中的表达有一定意义,不同治疗方法对其表达水平存在差异。

Objective： To observe the expression of fibroblast growth factors in rats by different therapeutic methods in TCM. Methods： The rats with liver cancer by DEN were randomly divided into the nomal group, the model group, the Nanfuding group, the group by Yiai Fuzheng Xingqi Huoxue method, the group by Qingre Jiedu method, the group by Xingqi Huoxue method and the group by Jianpi Fuzheng method. Affymetrix Rat 230A GeneChip and the relative technique were used to detect the different transcriptional expression of FGF between seven groups. Pubmed was used to search relevant references. Restilts： In Rat230A genechip there were 24FGFs,3 FGFRs and one FGFBP. Of 28 genes, there were15 genes whose expression levels in liver cancer were higher than those in normal tissues. In contrast, there were 13 genes whose expression levels in liver cancer were lower than those in normal tissues. The ratio of FGF13 that liver cancer tissue/normal liver tissue （ 123. 288/11.4） was 10.8,and the ratio of FGF7 that normal liver tissue / liver cancer tissue（55.2/4.224）was 13.1. In recent 10 years （Until 2006 - 1 - 1）, there were 857 literatures, of which only 49 were related with liver cancer,and only 3 genes whose FGF1 ,FGF2 and FGFR3 had been reported with liver cancer. Conclusion： Expression of FGFs in rats with liver cancer has a certain significance and different therapeatic methods have different effects on its expression level.