尖锐湿疣表皮CCL20及CXCR4的表达

Expression of CCL20 and CXCR4 in epidermal condyloma acuminatum lesions

目的了解尖锐湿疣(CA)表皮中CCL20及CXCR4的表达,并探讨它们对CA表皮朗格汉斯细胞的影响。方法使用Affymetrix寡核苷酸芯片,对3例CA表皮和3例正常表皮中的CCL20及CXCR4的mRNA表达进行检测。采用Westernblot法,检测3例CA表皮和3例正常表皮中的CCL20及CXCR4的蛋白表达。结果基因芯片检测到CCL20及CXCR4的mRNA在CA表皮中表达下调。Westernblot法检测到CCL20蛋白及CXCR4蛋白在CA表皮中的显著下调表达。结论CCL20及CXCR4在CA表皮中表达下调,并可能分别与CA表皮中朗格汉斯细胞的数目减少及回流障碍有关。

Objective To detect CCL20 and CXCR4 expressions in epidermis infected with condyloma acuminatum （CA） and normal epidermis and investigate the effect of their expressions on Langerhans cells in CA epidermis. Methods Gene expression of CCL20 and CXCR4 in 3 epidermal CA lesions and in 3 normal epidermis specimens were detected using Affymetrix oligonucleotide microarrays HG-U 133A 2,0, and the protein levels of CCL20 and CXCR4 in these specimens were measured by Western blotting. Results Microarray analysis revealed markedly down-regulated mRNA expressions of CCL20 and CXCR4 in the 3 epidermal CA lesions as compared with those in the normal specimens. Western blot analysis showed that the protein expressions of CCL20 and CXCR4 in the CA lesions were significantly lower than those in normal epidermis. Conclusion The protein and mRNA expressions of CCL20 and CXCR4 are markedly down-regulated in epidermal CA lesions, which may contribute to decreased number and backflow disturbance of Langerhans cells in these lesions.