用蛋白质芯片-飞行时间质谱仪分析精液不液化患者精浆中相关蛋白质群

Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry for proteomic analysis of seminal plasma in patients with delayed semen liquefaction

目的用蛋白质组学方法筛选精液不液化患者及正常生育患者精浆中的差异蛋白。方法通过蛋白质芯片-飞行时间质谱仪,对6例精液不液化患者的精浆和11例正常生育男性精浆样本进行检测,比较两组间蛋白质图谱表达差异。结果正常组与不液化组精浆比较,有19个蛋白表达存在差异,其中有6个蛋白P<0.01。结论精液不液化患者与正常生育患者精浆中存在较多差异蛋白质,差异蛋白质群的结构、功能变化是导致精液液化异常的主要原因;本研究对进一步探索不液化的病因及其临床治疗具有重要意义。

Objective To identify the differentially expressed proteins in seminal plasma between subjects with normal fertility and those with delayed semen liquefaction by proteomic techniques. Methods Semen samples of 6 patients with delayed semen liquefaction and 11 subjects with normal fertility （control group） were collected. Seminal plasma were separated and examined with surface-enhanced laser desorption/ionization time of flight mass spectrometry to compare the protein expressions between the two groups. Results Compared with normal control group, 19 proteins were differentially expressed in the patients including 6 proteins with significant differences between the two groups, with m/z of 8696.621, 9770.076, 9512.309, 10202.64, 2941.903, and 9617.759, respectively （P〈0.01）. Conclusion Screening ofdiffererxtially expressed proteins in seminal plasma may help understand the mechanism of delayed semen liquefaction for its potential clinical intervention.