中国华南地区汉族人群支气管哮喘患者ADAM33基因T_1位点多态性研究

Association between ADAM33 gene polymorphism and bronchial asthma in South China Han population

目的检测ADAM33基因T1位点多态性在中国华南地区汉族人群中的分布频率，探讨T1位点多态性与支气管哮喘的相关性。方法采用聚合酶链反应和限制性片断长度多态性及DNA测序的方法，对160例哮喘患者及95例健康人进行ADAM33基因T1位点多态性分析。结果在英国、美国、德国、韩国及中国华南地区的不同种族人群ADAM33基因T1位点等位基因频率的比较Х^2=9．085，P=0．059，差异无显著性。ADAM33基因T1位点3种基因型（TT、TC、CC）在哮喘组分布为129（80．6％）、27（16．9％）、4（2．5％），在对照组分布为90（94．7％）、3（3．2％）、2（2．1％），Х^2=10．955，P〈0．05，差异有显著性。哮喘组ADAM33基因T1位点等位基因T和C的频率分别为0．891、0．109；对照组ADAM33基因T1位点等位基因T和C分别为0．963、0．037。哮喘组与对照组T及C等位基因频率比较差异有显著性（Х^2=8．299，P〈0．05）。（4）进行单变量logistic回归探讨T1位点基因多态性与哮喘的关系，相对TT基因型而言，TC杂合型与TC＋CC均能显著增加哮喘发生的危险性，OR值（95％CI）分别为6．279（1．849-21．328）、4．326（1．620～11．550），P〈0．05。结论ADAM33基因T1位点基因多态性与中国华南地区汉族人群哮喘易感性相关。

Objective To investigate the association between the polymorphism of T1 locus allele in ADAM33 gene and bronchial asthma in South China Han population. Methods Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） and DNA sequencing were used to determine the polymorphism of T1 locus allele in ADAM33 gene in 160 unrelated patients with asthma and 95 unrelated healthy controls from South China Han population. Results No significant difference was found in T1 locus allele distribution frequency in populations of UK, US, Germany, Korea, and South China （Х^2=9.085, P=0.109）. The frequencies of the genotypes （TT, TC, CC） were 80.6% （n=129）, 16,9% （n=27） and 2.5% （n=4） in the 160 asthmatic patients and 94.7% （n=90）, 3.2% （n=3） and 2.1% （n=2） in the 95 controls, respectively, showing a significant difference in the distribution of the genotypes （TT, TC, CC ） between asthmatic patients and healthy controls （Х^2=10.955, P〈0.05）. The frequencies of the alleles （T, C） were 0.891 and 0.109 in the asthmatic patients and 0.963 and 0.037 in the controls, respectively, showing also a significant difference in the allele frequency between them （Х^2=8.299, P〈0.05）. The presence of C allele of ADAM33 gene T1 locus was found to be a greater risk factor in asthmatic patients than in the healthy controls. The odds ratio （OR） of TC and TC＋CC were 6.279 （1.849-21.328） and 4.326 （1.620-11.550）, respectively, with P value of 0.001 and 0.002, respectively, in comparison with TT genotype. Conclusion The polymorphism of T1 locus allele in ADAM33 gene is associated with the susceptibility to asthma in South China Han population.