BacT/Alert 3D系统与罗氏培养基分离分枝杆菌的比较

BacT/Alert 3D system versus Lowenstein-Jensen medium for isolation of mycobacteria from clinical specimens

目的评价BacT/Alert 3D系统从临床标本中分离分枝杆菌的价值。方法收集从2004年12月至2005年4月的临床标本共416份,每份标本接种BacT/Alert 3D系统的同时接种两支改良罗氏培养基和涂片作荧光染色。结果416份临床标本中,共分离出144株分枝杆菌,分离率为34.6%。BacT/Alert 3D系统分离130株,分离率为31.3%,罗氏培养基分离出126株,分离率为30.3%。BacT/Alert 3D系统平均阳性报告时间为是19.9 d,罗氏培养基平均阳性报告时间为26.4 d。BacT/Alert 3D系统的污染率为3.6%,罗氏培养培养基的污染率为5.8%。结论BacT/Alert 3D系统和罗氏培养基联合检测有助于提高检出率和降低污染率。

Objective To evaluate the value of BacT/Alert 3D system for isolation of mycobacteria from clinical specimens. Methods 416 specimens were collected from December 2004 to April 2005 and inoculated in BacT/Alert 3D system（0.5ml） and on two Lowenstein-Jensen（L-J） slants（0, 1ml each）, respectively. Smears were stained by anramine O. Results Of 416 clinical specimens, 144（34.6% ） were isolated Mycobacterium strains. Of these 144 strains, 130 strains were isolated by BacT/Alert 3D system, and 126 were isolated by L-J medium. The mean time of detection for BacT/Alert 3D system and L-J medium was 19.9 and 26.4 days, respectively. The overall contamination rates of BacT/Alert 3D system and on L-J medium were 3.6% and 5.8%, respectively. Conclusion Combination of BacT/Alert 3D system and L-J medium might raise isolation of mycobacteria and lower the contamination rate.