2型糖尿病肾病患者血清中TNF-α、NO与ET的水平变化及其临床意义

Detection of serum tumor necrosis factor-alpha,nitric oxide and endothelin levels in patients with type 2 diabetic nephropathy

目的:探讨2型糖尿病肾病患者血清肿瘤坏死因子α(TNF-α)、一氧化氮(NO)和内皮素(ET)的水平变化及其临床意义。方法:用ELISA法检测91例2型糖尿病患者血清TNF-α水平,NO与ET的水平分别用硝酸还原酶法和放射免疫法测定。结果:2型糖尿病肾病各组患者TNF-α和ET水平较对照组明显升高,其中ODN组最高(P<0.01)。而2型糖尿病肾病各组患者血清NO水平较对照组明显减少(P<0.01)。显性糖尿病肾病患者血清TNF-α和ET呈正相关;血清TNF-α和NO、ET和NO均成负相关。结论:TNF-α、NO与ET可能参与2型糖尿病肾病的发病及病程变化过程,检测患者TNF-α、NO与ET水平可作为判断预后、指导治疗的指标。

Objective：To observe the change of serum tumor necrosis factor - alpha （TNF - α）, nitric oxide （NO） and endothelin （ET） levels in patients with type 2 diabetic nephropathy （T2DN）, and evaluate its clinical significance. Methods： The level of TNF -α was measured by ELISA in 91 patients with T2DN. The levels of NO and ET were detected by nitrate reduction test and radioactivity immunoassay. Results ： The levels of TNF - α and ET in patients with T2DN were markedly higher than those of the normal group （ P 〈 0.01 ）, but the level of NO was lower than those of normal group （ P 〈 0.01 ）. There was a positive correlation between TNF - α and ET in patients with overt diabetic nephropathy those patients. Conclusion： TNF - α, disease activity. Detection of TNF - α, the clinical curative effects of drugs There was a negative correlation between TNF - α and NO, ET and NO in NO and ET are involved in the process of T2DN and closely related to the NO and ET levels may be helpful to observe the activity of the disease and