止血用ZSM-5沸石的细胞毒性评价

Cytotoxicity research of hemostatic agent ZSM-5 zeolite

目的：对ZSM-5沸石进行细胞毒性评价，探讨其作为急救止血材料的安全性。方法：参照我国医疗器械生物学评价标准：①采用L929细胞经沸石浸提液培养后，MTT法测定其相对增值率；②将L929细胞与沸石直接接触培养，不同时间观察细胞生长状态及细胞计数；③将L929细胞种植于沸石表面，扫描电镜观察其生长情况。结果：ZSM-5沸石性质稳定，细胞毒性程度为0～1级，符合我国医疗器械生物学评价标准。L929细胞与沸石直接接触培养生长良好，形态学无明显改变。结论：ZSM-5沸石细胞相容性好，符合我国医疗器械生物学评价标准。

Objective：To evaluate the cytotoxicity of ZSM -5 zeolite and investigate its reliability as first -aid hemostatic agent. Methods： According to Chinese evaluation standards on medical devices, experimental procedure was as follows： （a） L929 cells were cultured in the extraction botainted from zeolite to measure the relative growth rate by methyl- thiazolyl -tetrazolium （M3T） assay; （b） L929 cells were cultured directly with zeolite, the cell morphology was observed, and the cell number was counted; （c） the morphology of L929 cells cultured on the surface of zeolite was studied by scanning electron microscopy （SEM）. Results： The property of ZSM - 5 zeolite was stable and the toxic levels ranged from 0 to 1 grade. There were no signs of cytotoxicity induced by ZSM -5 zeolite, and the cell morphology was normal. Conclusion： The ZSM -5 zeolite has good cytocompatibility in vitro.