转录因子NFAT表达与大肠癌侵袭转移的关系

Correlation between transcription factor NFAT expression and invasion and metastasis in colorectal carcinoma

目的:研究活化T细胞核因子(NFAT)的表达与大肠癌侵袭及转移的关系。方法:以30例结直肠癌手术标本为研究对象,分别采用荧光实时定量PCR和免疫组织化学的方法检测肿瘤和切端组织中活化T细胞核因子基因的mRNA和蛋白表达水平,并结合相关临床病理资料进行统计学分析。结果:癌组织NFAT mRNA转录水平显著高于切端组织(P<0.01),NFAT mRNA转录水平与浸润深度、淋巴结转移情况和Dukes分期相关(P<0.05);癌组织NFAT蛋白表达水平显著高于远癌切端组织(P<0.01),NFAT蛋白表达水平与淋巴结转移情况(P<0.01)和Dukes分期显著相关(P<0.05);并且在肿瘤组织中NFAT mRNA和蛋白的表达水平呈正相关(P<0.01)。结论:NFAT在结直肠癌的恶性生物学行为中起重要作用,其高表达者肿瘤易于侵袭、转移,可作为判断肿瘤恶性程度、浸润转移的生物学指标;针对NFAT的干预有可能成为肿瘤治疗的重要手段之一。

Objective： To investigate the correlations between expressions of nuclear factors of activated T cells （NFAT） and invasion, metastasis in colorectal carcinoma（CRC）. Methods： The mRNA transcription and protein expression levels of NFAT in 30 panels of samples including tumor tissues and tissues of cutting side were assayed by real-time Quantitative PCR and immunohistochemical methods individually. All investigatory data combined clinico-pathological findings were statistically analyzed. Results： Both the mRNA and protein expression levels in tumor tissues were significantly higher than those in cutting side tissues. Significantly positive correlations between NFAT mRNA transcription level and neoplasia invasion, lymph node metastasis, Dukes＇ stage were respectively found. Significantly positive correlations between the protein expression level of NFAT and neoplasia lymph node metastasis, Dukes＇ stage were respective- ly found. There was correlation between NFAT mRNA and protein expression in tumor tissues. Conclusion： Overexpressions of NFAT in CRC suggest that NFAT might promote neoplasm angiopoiesis, then lead to tumor development, invasion and metastasis. Therefore, nant degree in the evaluation of CRC patients. NFAT could be a prognostic predictor of malignant degree in the evaluation of CRC patients.