E-cadherin在肝细胞癌中的表达及临床意义

Expression and clinical significance of E-cadherin in hepatocellular carcinoma

目的:研究上皮型钙黏蛋白(E-cadherin)的表达与肝细胞癌的临床病理参数间的相关性,探讨其与肝细胞癌发生进展的关系。方法:采用定量逆转录聚合酶链反应和免疫组织化学方法,检测30例肝细胞癌标本及癌旁组织中E-cadherin基因的mRNA转录水平及蛋白表达水平。结果:肝细胞癌组织中E-cadherin的mRNA转录水平及蛋白表达水平显著低于癌旁组织(P<0.01),与肝细胞癌的分化程度密切相关(P<0.05),E-cadherin的表达水平及转录水平间存在相关性(P<0.01)。结论:E-cadherin与肝细胞癌组织细胞异型性增加及浸润转移等相关,有助于判断患者预后情况。

Objective： To study the correlation between the expression of E-cadherin and the clinical pathological factors of hepatoeellular carcinoma and explore its role during the carcinoma process. Meth- otis： The quantitative-RT-PCR and immunohistochemieal methods were employed to determine the transcription level and expression level of E-cadherin in 30 cases of hepatocellular carcinoma, including the tumor tissue and the surgical margin tissue. Results： Both the transcription level and expression level of Eeadherin in the tumor tissue were significantly lower than those in the surgical margin tissue. And the results were correlated with the differentiation degree. Conclusion： E-eadherin is related with the malignant degree and the invasion and metastasis of hepatoeellular carcinoma and is helpful for the judgment of prognosis.