高效液相色谱-荧光法测定丙泊酚的血药浓度

Determination of propofol in plasma by HPLC with fluorescence detection

目的:建立高效液相色谱-荧光法检测丙泊酚血药浓度的方法。方法:采用饱和硫酸铵溶液作蛋白沉淀剂,以乙腈-水(70:30,含0.1%三氟乙酸)为流动相,流速1.0ml·min-1,荧光检测器检测。结果:丙泊酚血药浓度范围为0.1～10.0mg·L-1,(r=0.999 5);最低检测限为0.02mg·L-1;平均提取回收率为87.03%,RSD为2.54%;平均方法回收率为105.08%,RSD为3.81%;日内差异RSD为4.31%;日间差异RSD为7.54%。结论:本检测方法操作简便、灵敏度高、专一性强、结果可靠,可用于丙泊酚血药浓度监测和药物代谢动力学研究。

Objective： To develop a HPLC method for determination of propofol in human plasma. Methods： Saturated ammonium sulfate was used as deproteinsation solution to prepare sample. 20μl of supernatant was injected. The mobile phase was composed of acetonitrile-water （70：30, containing 0.1% trifluoroacetic acid） as rate of 1.0 ml. min^-1. The fluorescence detector was used. Results： Propofol had a good linear relationship within 0.1-10.0 mg· L^-1 （r = 0.999 5）. The limit of detection was 0.02 mg·L^-1 with an injection volume of 20μl. The extracting recovery of propofol was 87.03%, and the method recovery was 105.08%. The RSD of within-day and between-day were 4.31% and 7.54% respectively. Conclusion： The proposed method is simple, sensitive, accurate, reliable, and can be used for blood concentration monitoring and pharmacokinetic studies of propofol.