产金属β内酰酶铜绿假单胞菌的表型和基因型分析

Study on of metallo-β-1actamases in pseudomonas aeruginosa

目的:了解我院铜绿假单胞菌的耐药与流行病学状况,以便更好控制医院感染。方法:采用纸片法测定产金属B-内酰酶的PA,并用随机扩增多态性(RAPD)DNA基因分型方法对其进行PAPD基因分型。结果:24株耐亚胺培南PA通过纸片法检测确定产金属β-内酰酶19株,不产金属β-内酰酶5株,产金属B-内酰酶检出率80%;PAPD法共得PAPD十型,分型率为100%。有3例感染个体出现相同基因型,有1例病人在不同时期出现两种基因型。结论:纸片法检测能快速简便检测出金属β-内酰酶,随机扩增多态(PAPD)DNA基因分型,能满足对PA进行分子流行病学调查。

Objective：To monitor the rug resistance and epidemiology state of pseudomonas aeruginosa （PA）isolated clinical patient. Methods：antimicrobial sensitivity tests were performed hy K-B method and metallo-β-lactamases-producing PA was genotyped by the randomly amplified polymorphic （PAPD）assay. Result：Among24 strains resistant to imipenem.19 （8%）strains produced metal enzyme which could be distingurished into 10 kinds of PAPD type.the type ability was 100%. There was same gene type strain. Isolated from three patient and there were two kinds of gene types. Among 2 strains isolated from the same patient at the different time. Conclusions：the K-B method could be used to determine metal enzyme, rapidly and conviently. And PAPD could to be used in the molecular epidemiological study of PA.