基因芯片技术筛选吉西他滨诱导胰腺癌Panc-1细胞凋亡相关基因

Gene microarrays in detecting molecular mechanisms of gemcitabine-mediated apoptosis on human pancreatic carcinoma cells panc-1

目的研究抗癌药吉西他滨（Gemcitabine）诱导胰腺癌Panc-1细胞凋亡的相关基因。方法应用基因芯片技术检测50．33μg／L Gemcitabine作用于胰腺癌PANC-1细胞48h后凋亡基因表达的变化。结果Gemcitabine抑制细胞表达14种凋亡相关基因，包括IRF-4、Scotin、14—3—3gamma、PDE4、GRPS、TID1、AFP、STAT5、Sox-4、c-Rel、MYCN、Htt、CatD、Gd。相反，gemcitabine促进了3种凋亡相关基因的表达，包括CyclinB1、ASK1、AKT2。结论Gemcitabine诱导胰腺癌Panc—1细胞凋亡通过非依赖caspaseASK1途径。

Objective To study the molecular mechanisms of antitumor agent Gemcitabine on human carcinoma cells. Methods Gene expression profile was identified by cDNA microarrays on pancreatic carcinoma Panc-1 cells exposed to Gemcitabine（50.33mg/L） for 48hr. Results Gemcitabine-treated cells decreased expression of seventeen apoptotic genes. In contrast, these treated cells overexpressed three apoptotic genes. Conclusion It displayed that the apoptotic pathway could be caspase-independent ASK1 pathway.