细胞周期依赖的蛋白激酶抑制剂Olomoucine对脊髓损伤后硫酸软骨素蛋白多糖表达的影响

Effects of cell cycle inhibitior olomoucine on the expresion of chondroitin sulphateproteoglycans after spinal cord injury

目的探讨细胞周期抑制剂Olomoucine对脊髓损伤后硫酸软骨素蛋白多糖（CSPGs）表达的影响及意义。方法建立大鼠脊髓半切损伤模型，随机分为假手术组、损伤对照组和Olomoueine干预组，采用免疫印迹分析脊髓损伤后CSPGs的表达；应用免疫荧光技术检测损伤区域胶质纤维酸性蛋白（GFAP）和CSPGs的表达；采用改良Gale联合评分法对大鼠瘫痪后肢进行运动功能评估。结果脊髓损伤后星形胶质细胞明显活化增殖，GFAP和CSPGs的表达显著高于假手术组（P〈0．05），Olomoucine干预可有效下调GFAP和CSPGs的表达（P〈0．05），改善瘫痪后肢的运动功能（P〈0．05）。结论Olomoucine可通过调控细胞周期，抑制胶质细胞的活化增殖和胶质瘢痕的形成，减少抑制因子CSPGs的表达，促进脊髓损伤后瘫痪后肢的运动功能恢复。

Objective To explore the effects of cell cycle inhibition on the expression of chondroitin sulphateproteoglyeans （CSPGs） after spinal cord injury （SCI） . Method SCI model was produced by hemisection technique. Rats were randomly divided into sham group, SCI group and olomoucine treatment group. The expression of glial fibfillory acidic protein （GFAP） and CSPGs were observed by The level of CSPGs was measured by Western blot analysis. The motor function of hindlimb was determined by combine behavioral scale （CBS）. Results Spinal cord injury resulted in astrocytic acitivation, and proliferation and increased expression of CSPGs, leading eventually to the formation of glial scar. Olomoucine treatment astrocytic proliferation, down-regulated expression of GFAP and CSPGs proteins （P 〈 0.05）, and improved hindlimb motor function. Conclusions Olomoucine could improve motor function, partially through inhibition of glial scar formation and CSPGs expression.