时间分辨荧光技术在乙肝病毒标志物定量检测中的应用

Application of TRFIA in HBV-M quantitative detection

目的比较时间分辨荧光免疫分析技术(TRFIA)、酶联免疫吸附试验(ELISA)两种方法在测定乙型肝炎病毒标志物(HBV-M)时结果的符合程度,了解TRFIA测定HBV-M的可行性。方法分别用TRFIA和ELISA测定120份随机血清样本乙肝两对半并计算结果符合率。结果TRFIA、ELISA法测定120份随机样本HBV-M结果符合率较高,经配对资料的χ2检验,P>0.05,无显著性差异。结论TRFIA是定量检测HBV-M的方法,它可为临床诊断、临床疗效的观察及注射疫苗提供科学依据。

Objective To compare the consistence between TRFIA and ELISA in HBV-M determination and find out the feasibility of FRFIA method in HBV-M measurement. Methods The five items of HBV in 120 randomized samples were determined by TRFIA and ELISA respectively.The consistence of results was calculated. Results HBV-M measurement results in 120 randomized samples by TRFIA and ELISA were consistent. There was no obvious difference by matching data test toХ^2,P〉0.05.Conclusions TRFIA was a quantitative detection method to HBV-M, it could provide science base for diagnosing, evaluating the clinical treatment effect, and injecting hepatitis B vaccine.