表浅性与浸润性膀胱癌基因表达谱的比较

Identification of gene expression patterns in superficial and invasive human bladder cancers

目的比较人表浅性与浸润性膀胱癌基因表达谱的差异。方法应用基因芯片技术对表浅性膀胱癌和浸润性膀胱癌的 RNA 进行检测分析。得到芯片灰度扫描图,通过图像处理软件GenePix Pro 3.0对图像进行分析,得到表达差异基因的相应数据,根据差异基因的类型及膀胱癌的特点分析数据。结果按显著差异基因标准,从13939条基因中筛选出显著差异表达基因:表浅组714条,其中480条下调,234条上调;浸润组470条,其中302条下调,168条上调。在显著差异基因中,下调基因的数量比上调基因多近1倍,免疫相关基因在浸润性膀胱癌中下调最明显。结论浸润性和表浅性膀胱癌的基因表达谱具有许多共同特点,但表浅性膀胱癌的差异表达基因较浸润性膀胱癌更多。免疫相关基因在浸润性膀胱癌中缺失较表浅性膀胱癌严重。

Objective To study the difference of gene expression patterns in invasive and superficial human bladder cancers. Methods cDNA microarray was used to detect the RNA in 12 samples of invasive and superficial human bladder cancers and 6 samples of normal bladder tissues as control group obtained during operation. Then, the scanogram was analyzed by applying the image processing software GenePix Pro 3.0 so as to obtain the relevant data of genes. FinaUy, we analyzed these data by some knowledge about the type of genes and the characteristic of human bladder cancer. Results There were 714 genes with remarkable differences in the samples of superficial bladder cancer, of which 480 were downregulated and 234 were up-regulated; and there were 470 genes with remarkable differences in the samples of invasive bladder cancer, of which 302 were down-regulated and 168 were up-regulated. The number of down-regulated genes in the samples of superficial bladder cancer was two times that of up-regulated genes. Most of the up-regulated genes were metabolism, cell signal transduction, DNA combination and transcription, protein translation and synthesis, and development-related genes; and most of the downregulated genes were immunization-related genes. The down-regulation of immune-related genes was more remarkable in the invasive bladder cancer than in the superficial tumor. Conclusion There are many same features in gene expression spectrum in invasive and superficial human bladder cancers. However, the number of gene phenotype of the superficial human bladder cancer is more than that of invasive one. The loss of immunization-related genes is more severe in the invasive human bladder cancer than in the superficial tumor.