黄芪提取物保护Aβ致海马神经元损伤

Protective effect of extract of astragalus against injury induced by amyloid peptide in hippocampal neuron

目的探讨黄芪提取物在Aβ所致海马神经元损伤中的作用。方法通过对孕18d胎鼠的海马神经细胞培养,观察Aβ对神经细胞的毒性及黄芪提取物的保护作用。采用LDH法和MTT法检测细胞活力,LSCM测胞质钙离子浓度的变化及TUNEL法测细胞凋亡。结果Aβ与细胞孵育后,细胞活力明显下降,并呈时间和剂量依赖性,同时细胞内钙显著上升,细胞出现凋亡的典型的形态学特征。20、40μg·L-1黄芪提取物能明显提高细胞活力、降低胞质钙离子浓度、减少凋亡的细胞数。结论黄芪提取物对Aβ所致海马神经元损伤具有一定的保护作用,可能与其降低海马神经元胞质钙离子有关。

Aim To study the effect of EA on the injury induced by β-amyloid protein （Aβ） in primary cultures of rat hippocampal neurons. Methods The protective effect of EA on Aβ25-35 induced neurons injury was observed by LDH release rate, MTT, LSCM and TUNEL..Results A25-33 could induced cell death in rat primary hippocampal neurons. Four hours pretreat-ment with 20 mg· L^-1, 40 mg·L^-1 EA exerted the protective effect on rat primary hippocampal neurons from Aβ25-35 induced injury. Conclusion EA had protective effects against injury induced by Aβ25-35 in rat primary hippocampal neurons to some certain, which probably related with decreasing the level of calcium.