摘要
目的:观察内毒素(ET)致兔急性肺损伤(ALI)时肺组织中过氧化物酶体增殖物激活受体γ(PPAR-γ)蛋白表达及美洛昔康的影响,探讨美洛昔康对兔急性肺损伤干预的机制。方法:将24只日本大耳白兔随机分为对照组、致伤组、美洛昔康干预组。用ET(700μg/kg)一次性静脉注射方法复制兔ALI模型,应用美洛昔康(2.5mg/kg)进行干预。采用免疫组织化学技术和Westernblot方法检测肺组织中PPAR-γ蛋白的表达。结果:PPAR-γ蛋白表达在致伤组显著低于对照组(P<0.01),美洛昔康干预组显著高于致伤组(P<0.01)。结论:PPAR-γ蛋白表达在ALI时下调。美洛昔康可促进PPAR-γ蛋白的表达,在一定程度上对兔内毒素性ALI产生保护作用。
Objective： To investigate the expression of peroxisome proliferator- activated receptor-γ （PPAR-γ）protein in lungs of rabbits with acute lung injury induced by endotoxin （ET） and the protective mechanism of meloxicam. Methods： Twenty four Japanese flap-eared white rabbits were randomly assigned to three groups： control group, ET -treated group and meloxicam -treated group. Rabbit ALI models of rabbits were replicated with intravascular ET injection （700 g/kg weight）, meloxicam （2.5 mg/kg weight） was intravascularly injected as treatment group. Using immunohistochemistry and Western blot analysis, we measured PPAR-γ protein in each group. Results：PPAR-γ protein expression in ET-treated group was significantly lower than that in control group （P 〈 0.01 ）, and PPAR-γ protein expression in meloxicam-treated group was significantly higher than that in ET-treated group（P 〈0.01 ）. Conclusion： The down-regulation of PPAR-γ protein may be involved in ALI. Meloxicam can upregulate PPAR-γ protein expression, which posseses protective effects on ALI induced by ET.
出处
《军医进修学院学报》
CAS
北大核心
2007年第2期94-96,共3页
Academic Journal of Pla Postgraduate Medical School
