6种市售低分子肝素的分子特性及生物活性的比较

Compare of Molecule Characteristics and Bioactivity of Low-Molecular-Weight-Heparin Products in Market

目的考察国内市售低分子肝素产品间的差异,研究其结构特征、相对分子质量及其分布与生物活性的关系。方法采用分光光度法测定产品的特征组份的含量,生物显色法测定抗凝血的生物活性,凝胶渗透色谱法测定相对分子质量及其分布。将这些参数进行统计学分析。结果6种低分子肝素产品间分子的特能组成,相对分子质量及其分布和生物活性上均显示极显著性差异。相关性研究表明,分子中葡萄糖醛酸和葡萄糖胺的含量与低分子肝素相对分子质量、抗-Xa活性、抗-Ⅱa活性呈负相关;低分子肝素相对分子质量与抗-Ⅹa活性、抗-Ⅱa活性呈正相关;而硫酸基含量与低分子肝素相对分子质量、抗-Ⅹa活性、抗-Ⅱa活性呈正相关。结论每一个低分子肝素产品间都存在着明显的差异,具有不同的特点,临床使用中应按指征个体化给药。

OBJECTIVE To investigate the difference between low-molecule-weight-heparin products in market, and to study the relationship among their special structure, molecular weight and bioactivity. METHODS The contents of special structure were determined by spectrnphotometry; the bioactivity of anticoagulation was obtained by bio-coloration; the molecular weight and its distribution were measured using gel permeation chromatography; these parameters were statistically analyzed. RESULTS There is exactly significant difference among special structure, molecular weight and bioactivity of tile six low molecule heparin products in market. There is negative correlation between the content of glycuronic acid, aminoghleose and molecular weight, anti- X a activity ,anti-Ⅱa activity. There is pnsitive correlation between molecular weight and anti-X a activity ,anti-Ⅱa activity. While there is positive correlation between the quantity of sulphuric group and anti-X a activity ,anti-Ⅱa activity. CONCLUSION Every low molecule heparin product has its own fealure, and they should be used individualized in clinic.