摘要
目的观察角膜AQP1含量变化与死亡时间的关系。方法50只家兔随机分为10组,以空气栓塞方法致死,于死后不同时间取出角膜。应用免疫组织化学方法观察角膜AQP1含量变化,并采用图像分析方法测定AQP1阳性面积、积分光密度、平均光密度、平均灰度等参数,对各参数与死亡时间的关系进行统计学分析。结果死后84h内,角膜AQP1呈阳性着色,死后96h未见着色。图像分析结果显示,随着死后时间的延长,角膜AQP1各参数呈逐渐下降的趋势,并获得了上述各参数与死亡时间变化关系的回归方程。结论家兔死亡后角膜AQP1的含量呈现出规律性变化,其参数阳性面积、积分光密度、平均光密度、平均灰度等可以作为推测死亡时间的参考指标。
Objective To observe the relationship between the changes of corneal aquiporin-1 （AQP1） and postmortem interval （PMI）. Methods 50 healthy rabbits were divided randomly into 10 groups according to the time of 0h, 6h, 12h, 24h, 36h 48h, 60h, 72h, 84h and 96h after the death caused by air embolism and the cornea was dissected. The corneal AQP1 was observed by the method of immunohistochemical staining and analyzed by a image analyze system, the parameters of positive area （A）、integral optical density（IOD）、average optical density（AOD） and average gray（AG） was used to measure the content changes of corneal AQP-1 after different PMI. Results The positive reaction of corneal AQP1 was showed a time-dependent decrease until 84h after death, and the parameters of A, IOD, AOD and AG were also showed a decline regularly. Conclusion The content of corneal AQP1 was changes regularly after death, and the parameters of A, IOD, AOD and AG could be used as new indexes for estimating the PMI.
出处
《刑事技术》
2007年第2期15-17,共3页
Forensic Science and Technology
