小麦抗秆锈突变系龙辐03D51的筛选及其抗病性的遗传分析与RAPD标记

BREEDING WHEAT MUTANT LONGFU 03D51 WITH RESISTING STEM RUST AND GENETIC ANALYZATION OF RESISTING DISEASE AND RAPD MAKER

以龙6239幼胚为外植体,利用辐射诱变和组织培养相结合的方法,选育出突变系龙辐03D51,经秆锈接种鉴定,发现其对优势小种21C3CPH免疫,而亲本龙6239对21C3CPH高度感染。遗传分析表明,抗病性由显性单基因控制。该突变系还具有其亲本的优质、高产特点,已成为优异的后备品系。在RAPD检测中,所用的60个随机引物中有3个引物在龙辐03D51和其亲本龙6239中具有多态性,引物E07、E11、E17在龙辐03D51中分别扩增出380bp、700bp和600bp的特异带,初步认为这些谱带可能与秆锈抗性有关。

Longfu 03D51 was bred using Long 6239 immature embryo as explants through radiation mutagenesis breeding coupled with tissue culture technique. By artificial inoculation and field breeding identified Longfu 03D51 was identified with high resistance to dominant strain 21C3CPH, good quality and high yield. Genetic analysis suggested that stem rust was controlled by a single gene. The DNA polymorphism on Longfu 03D51 and its parents were studied by random amplified polymorphic DNA （RAPD）,3 primers out of 60 RAPD primers were found polymorphism between the Longfu 03D51 and its parent Long 6239, and 3 stable polyphymorphic bands amplified in Longfu 03D51 with the size of 380bp, 700bp, 600bp by random primer E07, E11, E17, respectively. Genetic and RAPD analysis indicated that 3 RAPD primers E07, E11, E17 might be linked to the gene of stem rust resistance.