扩繁条件对两种AMF菌剂接种势的影响

Effected on the Inoculum Potential of Two Kinds of Arbuscular Mycorrhizal Fungi Inoculum by the Propagation Conditions

温室盆栽试验,分别以4种宿主植物和6种基质对两种AMF菌剂AM-1(Glomus intraradices)和AM-2(Glomus spp.)进行了扩繁研究。通过计算菌剂接种势,评价不同扩繁条件下获得菌剂的质量。结果表明,白三叶草和烟草可以作为两种菌剂良好的扩繁宿主,得到菌剂的接种势最高,玉米和高粱次之。当以白三叶草为宿主时,单一蛭石可以作为两种菌剂良好的扩繁基质,得到菌剂的接种势最高,珍珠岩次之,其它基质较差,基质中草炭的含量会影响菌剂扩繁效果。

In greenhouse condition, arbuscular mycorrhizal fungi Glomus intraradices （AM-1） and Glomus spp. （AM-2） were propagated in symbiosis with four host plant species, or with six kinds of substrates, in pot culture. The quality of the resultant inoculums under different test conditions were evaluated by calculating their inoculum potential （IP） . Results indicated that white clover and tobacco were the suitable hosts for the propagation of AM-1 and AM-2, and the inoculums derived from them had higher IP, followed by maize and grain sorghum. With white clover as host, the vermiculite was the best substrate to producing the high quality inoculums in terms of IP, followed by the perlite. In contrast, grass charcoal showed negative effect on the propagation of AM-1 and AM-2.