Zwittermicin A的分离纯化及其稳定性的初步研究

Study on Separation and Purification of Zwittermicin A and its Stability

通过离子交换法,用D113树脂对苏云金芽孢杆菌库斯塔克亚种D1-23发酵液进行初步提纯,经HPLC-MS纯化和制备,得到2.89mg Zwittermicin A纯品。离子交换第1次冲洗液NH4H2PO4的最适浓度为5mmol/L;第2次冲洗液CH3COONH4的最适浓度为30mmol/L,梯度冲洗pH值为8.0～9.5;Zwittermicin A的总收得率达93%。Zwittermicin A的热稳定性和pH值稳定性实验表明,Zwittermicin A在100℃下的半衰期为48.22min;在pH值3.0～12.0范围内pH值越低,Zwittermicin A的稳定性越强。

Zwittermicin A was purified by ion exchange resin and HPLC from supematants of Bacillus thuringiensis, subsp, kurstaki strain D1-23 cultivation. 2.89mg pure Zwittermicin A was acquired, proved by HPLC-MS. Results show that the optimized wash concentration of NH4H2PO4 is 5mmol/L at first step. Next step CH3COONH4concentration is 30 mmol/L, the gradient pH is 8.0 -9. 5. Totally 93% Zwittermicin A can be reserved with ion exchange resin. The temperature and pH stability experiments show the half life of Zwittermicin A is 48. 22 minutes in 100℃, and it is more stable in lower pH in pH 2.0 - 12.0.