大鼠死后肝脏磷酸果糖激酶-2含量的变化

Postmortem Changes of Liver Phosphofructokinase-2 Level in Rats Following Different Causes of Death

目的分析不同死亡时间和死亡原因大鼠肝脏组织内磷酸果糖激酶-2(PFK-2)含量的变化。方法大鼠105只随机分为三组,以不同方式(失血、窒息、断颈)处死。分别于死后0、1、2、4、8、12和24h取大鼠肝脏组织,免疫组化和图像分析技术测定大鼠PFK-2变化。结果三组不同死亡原因大鼠肝脏组织内PFK-2的含量随死亡时间延长呈规律性减弱趋势。结论大鼠肝脏组织内PFK-2的变化可以反映死亡时间的变化趋势。

Objective To study the changes of liver phosphofructokinase-2 （PFK-2） level at different postmortem intervals as well as due to different causes of death. Methods One hundred and five rats were randomly divided into 3 groups and the rats were sacrificed by either bleeding, suffocating, and neck breaking, respectively. The content of liver PFK-2 at 0, 1, 2, 4, 8, 12 and 24 hours following death were studied using immunohistochemishtry and image analysis. Results PFK-2 content of the rat＇s liver in all 3 groups showed a linear decrease at different postmortem intervals with no significant statistical differences found between the different groups. Conclusion PFK-2 level in rat liver appears to decrease linearly in correlation with prolonged PMI.