肌红蛋白降解与人体死亡时间的相关性

Relationship Between the Degradation of Myoglobin and Postmortem Interval

目的检测人体死亡后骨骼肌中肌红蛋白的降解水平,探讨其与死亡时间的关系。方法分别于人体死后0、4、8、12、24、36、48、60、72h取骨骼肌,置于室温25℃,在不同时间点抽提蛋白质,用Western Blot方法检测肌红蛋白,随后利用生物电泳图像分析软件分析。结果肌红蛋白随死后时间逐步降解。结论肌红蛋白降解的检测可用于推断死亡时间。

Objective To observe the degradation of myoglobin in China） skeletal and cardiac muscles after death and to explore its usefulness in estimation of postmortem interval. Methods Human skeletal and cardiac muscles were collected at 0, 4, 8, 12, 24, 36, 48, 60, and 72 hours after death and stored at 25℃. The protein was extracted and examined and analyzed by Western blot and imaging technique. Results Myoglobin showed a continuously postmortem degradation in both skeletal and cardiac muscles. There was no observed difference in degradation between the two tissues. Conclusion Changes could be a useful parameter for estimation of the postmortem interval.