摘要
利用黑曲霉产酶发酵培养基制备β-葡萄糖苷酶,再利用β-葡萄糖苷酶水解大豆异黄酮粉制备异黄酮苷元。研究结果表明,较优产酶发酵培养基的C/N为6∶4,加水量1.4倍,培养基中不添加诱导物。水解500 mg40%大豆异黄酮粉的最佳条件为:加酶量100 U,水解温度50℃,水解时间1 h。
The soybean isoflavones was hydrolyzed by β- glucosidase obtained by fermentation with Aspergillus niger. The results showed that the better fermentation conditions were : ratio of C to N 6 : 4, water dosage 1.4 times of the total weight of carbon and nitrogen sources, no inducement in medium. The optimal hydrolysis conditions were: 500 mg soybean isofavones with the content of 40%, enzyme dosage 100 U,temperature 50 ℃ ,time 1 h.
出处
《中国油脂》
CAS
CSCD
北大核心
2007年第4期59-61,共3页
China Oils and Fats
基金
哈尔滨市科技攻关计划项目(2005AA6CN081)