食用油中黄曲霉毒素B_1免疫亲和检测技术研究

Study on the immunoaffinty technology for determining aflatoxin B_1 in edible oil

采用国内自主研制的黄曲霉毒素专用荧光快速检测仪及配套的免疫亲和试剂盒建立一套快速的免疫亲和检测方法,对食用油中的黄曲霉毒素B1进行测定。用10 mL石油醚和15 mL70%甲醇对5.0 g样品进行初步提取,过滤后,萃取液用免疫亲和微柱纯化富集,洗脱液放入黄曲霉毒素专用荧光快速检测仪中自动读取结果。实验结果表明,该方法的直接读数的线性范围为0.3-25μg/kg,R2=0.999 5,方法的平均回收率为87.11%,相对标准偏差为1.44%-6.33%,最低定量浓度为0.3μg/kg;应用于食用油样品AFB1检测,方便快速,安全准确,测定全过程所需时间可以控制在30 m in左右,检测成本低于其他仪器方法。

An immunoaffinity fast test technology was established for determining aflatoxin B1 in edible oil by homemade special fluorescence fast test apparatus for aflatoxin and correlative immunoaffinity reagent kit. Samples weighed 5.0 g were simply extracted by 10 mL petroleum ether and 15 mL 70% methanol solvent. After filtered,the extract was purified and enriched by immunoaffinity micro - column. The eluate was tested in the special fast test apparatus with the results being read directly. The readable linear of the method was 0.3 - 25 μg/kg with R^2 0. 999 5, and the average recovery was 87.11% with RSD 1.44% - 6.33%. The limit of quantitative detection was 0.3 μg/kg. The immunoaffinity technology for AFB1 in edible oil was developed with special fast test apparatus and correlative immunoaffinity reagent kit. Aflatoxin B1 in the edible oil was detected fast and conveniently with high accurateness and security. The time of whole test process was consumed about 30 minutes. The cost of detection was lower than other instruments and methods.