摘要
全血细胞分析系统成套设备作为预防、诊断疾病及肿瘤等有效技术手段。准确一致的诊断结果是所有医疗活动的核心。因此,临床使用血液细胞分析仪时,需要进行室间、室内质量控制,通过全血质控物来确认仪器的各项测定参数是否偏倚,以监控其测定结果的准确性和稳定性。但全血质控物为血液制品,因此血液中细胞成分不易稳定。使得在实际应用中并不能起到质量监控作用。笔者通过大量实验研究发现:除了在制备质控物的过程中加入适量的营养物质,以延长质控物的保存期限外,在质控物中添加适量的化学试剂-醛类物质,选择恰当浓度及最佳醛化条件来处理全血细胞是解决上述问题的关键所在。本试验选用与新鲜血液比例不同的醛化剂混合液,对全血细胞进行醛化,加入一定量的细胞保存液后,置于37℃条件下保存,通过观察得出结论:从性能稳定与成本角度、安全角度综合考虑,醛化试剂与新鲜血液采用我们优选的比例时,在37℃条件下,各参数可以稳定较长时间,这足以满足产业与临床的需求。
Equipment in assembled state of Hematology analyzer is a efficient technological instrument which prevent, diagnose, cure infection or tumour.Hematology analyzer is the most commonly-used equipment in all kinds of hospital laboratories. For checking the constancy of performance of the instrument and repeatability and reproducibility of mensurate result, whole blood quality control materials is utilised in internal quality control before use hematology analyzer.
Because of whole blood quality control materials is hemal materials,so its period of validity is very short, variations may occur with any time. So whole blood quality control materials were prepared by partial fixation with aldehydes in different proportionto whole blood.So as to not only fixup red cell,white cell and blood platelet,but also red blood can be haemolyzed by hemolytic agent. Accordingly, the result during periods can be stable.
Assess result of experiment, we can confirmed that: think over stability of capability, cost and safety, when we choose a appropriate proportion, although temperature is 37℃,the ingredient of whole blood quality control material can be stabilization in 76 hours,which can satisfaction
出处
《生命科学仪器》
2007年第3期35-38,共4页
Life Science Instruments
关键词
全血细胞分析
全血质控物
醛化剂
血细胞分析仪
Whole blood cell analysis
Whole blood quality control materials
Hydroformylation reagent
Hematology analyzer