重庆何首乌遗传多样性的SRAP研究

SRAP study on genetic diversity of Radix Plygoni Multiflori in Chongqing

目的：采用新型分子标记SRAP对重庆地区何首乌资源进行遗传多样性研究，为何首乌种质资源的分类、鉴定和选种打下良好的理论基础。方法：选择重庆各地区形态差异较大的16份何首乌材料进行SRAP多态性分析，利用DPSv3．01和UPGMA法进行聚类分析，构建遗传系统树。结果：从155个引物组合中筛选出104个多态性组合，共扩增产生了250个多态性位点，聚类分析结果显示，16份材料可以分为2大类和1个特异类，Nei＆Li相似系数在0．23-0．99，平均遗传距离为0．44。结论：何首乌资源遗传关系大体上符合地域差异，但也不能完全按照地理位置来判定它们的亲缘关系，应有少量的变异，SRAP技术研究中药资源多样性具有极大的优越性和可行性．

Objective： To detect the polymorphisms of Radix Plygoni Muhiflori in Chongqing by means of a new marker system SRAP. Method： Different shaples of Radix Plygoni Multitiori from major production areas were collected. The SRAP was used to asses divergence among 16 populations. The data were analyzed using unweighted pairgroup method, based on arithmetic averages （ UPG- MA） bootstrap analysis. Cluster analyses was performed by using DPSv3.01 software, the alkaloid was extracted from P. ternate with chlorolform. Result： 104 combinations generated 250 polymorphie bands, the cluster analysis indicated that 16 materials could be dis- tinguished into two main groups and one special type, Nei＆Li similarity coefficient ranged from 0. 23-0. 99, and the average distance is 0. 44. Conclusion： The results of the study showed a potential application of SRAP fingerprinting for identification of Radix Plygoni Muhiflori.