吡虫啉在麻黄中残留的分析方法

Study on residue analysis method of imidacloprid in Ephedrae sinica

目的：研究建立吡虫啉在麻黄中残留的液相分析方法。方法：样品经二氯甲烷提取，色谱柱净化，采用十八烷基键合硅胶柱，流动相为甲醇-水（20：80）；检测波长270nm。结果与结论：该方法的最小检出量为0．4×10^9g，最低检出质量分数为0．02mg·kg^-1，平均回收率为85．37％～90．65％，RSD为2．23％～3．45％；该方法的灵敏度、精密度和准确度符合农药残留分析的要求。

Objective： To establish residue analysis method of imidacloprid in Ephedra sinica by HPLC. Method： Imidacloprid was extracted with dichloromethane, cleaned up with chromatography column, then separated on Spherisorb C18 column by using Methanol-water （20： 80）, detected at 270 nm. Result and Conclusion： The limit of detection （LOD） and limit quantification （LOQ） were 0. 4×10^9g and 0. 02 mg ·kg^-1, mean recovery and related standard deviation （RSD） were 85. 37% -90. 65% and 2. 23%- 3.45%. It is concluded that the method could satisfy the pesticide residue analysis demands in sensitivity, accuracy and precision.