摘要
目的:通过应用HPLC—ECD分离测定猕猴血清中单胺类递质,验证其与经前期综合征(PMS)肝气逆证的内在关系,筛选科学有效的检测方法。方法:采用ZORBA×SB-C18色谱柱(4.6imm×250mm,5μm),流动相:乙酸钠100mmol·L^-1,柠檬酸85mmol·L^-1,正二丁胺0.4mmol·L^-1,辛烷基磺酸钠1.5mmol·L^-1,乙二胺四乙酸(EDTA)0.2mmol·L^-1,甲醇18%,抽滤。流速0.9mL·min^-1,量程1μA。结果:猕猴血清中去甲肾上腺素(NE),肾上腺素(E),多巴胺(DA)和5-羟色胺(5-HT)回收率依次为97.0%,97.8%,99.5%,100.3%,RSD0.22%-0.93%,重复性较好。结论:该方法操作简便、快速、准确,是检测猕猴血清中单胺类递质检测的一种理想方法。
Objective: To develop an HPLC-ECD for the determination of monoamine transmitters in serum of macaque. Method: The analysis was carried out on a ZORBAX SB-C18 column (4. 6 mm× 250 mm,5 μm) eluted with a mobile phase of metha- nolwater ( 18: 82) at a flow rate of 0. 9 mL · min^-1. Result: The recoveries of NE, E, DA, 5-HT were 97.0%, 97. 8%, 99. 5%, 100. 3%, RSD was 0. 22%-0. 93%, and the repeatability was good. Conclusion: The results prove that the method is simple, fast, accurate and can be used to determine simultaneously the concentration of monoamine transmitters in serum of macaque.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2007年第8期703-705,共3页
China Journal of Chinese Materia Medica
基金
国家自然科学基金资助项目(30572291)