双酚A与人血清白蛋白作用特征的热力学研究

Thermodynamic Studies on the Binding Feature between BPA and Human Serum Albumin

利用荧光光谱和吸收光谱法研究了双酚A与人血清白蛋白结合反应的特征,实验结果表明,双酚A对人血白蛋白有较强的荧光猝灭作用,根据荧光猝灭数据和非辐射能量转移机理,由Sterm-Volmer和Lineweaver-Burk方程处理实验数据,得到了结合反应的结合常数、结合位置和结合过程的基本热力学参数。

The characteristics of the binding reaction of biphenol A （BPA） with Human Serum Albumin （HAS） was investigated by fluorescence and absorption spectra. The BPA exhibits a strong quenching effect on the fluorescence of HSA,through a nonradiative energy transfer. The data of fluorescence quenching were analyzed according to Sterm-Volmer and Lineweaver-Burk equation,the binding constant of the reaction, the position of binding and the basic thermodynamic parameters of the binding process were obtained, respectively.