摘要
利用荧光光谱和吸收光谱法研究了双酚A与人血清白蛋白结合反应的特征,实验结果表明,双酚A对人血白蛋白有较强的荧光猝灭作用,根据荧光猝灭数据和非辐射能量转移机理,由Sterm-Volmer和Lineweaver-Burk方程处理实验数据,得到了结合反应的结合常数、结合位置和结合过程的基本热力学参数。
The characteristics of the binding reaction of biphenol A (BPA) with Human Serum Albumin (HAS) was investigated by fluorescence and absorption spectra. The BPA exhibits a strong quenching effect on the fluorescence of HSA,through a nonradiative energy transfer. The data of fluorescence quenching were analyzed according to Sterm-Volmer and Lineweaver-Burk equation,the binding constant of the reaction, the position of binding and the basic thermodynamic parameters of the binding process were obtained, respectively.
出处
《光谱实验室》
CAS
CSCD
2007年第2期161-164,共4页
Chinese Journal of Spectroscopy Laboratory
基金
广西壮族自治区卫生厅自筹经费立项课题
课题编号:Z2006307
关键词
双酚A
人血清白蛋白
热力学参数
BPA,Human Serum Albumin,Thermodynamic Parameters.