高效毛细管电泳法测定膝沟藻毒素2和3

Analysis of gonyautoxin-2&3 by high performance capillary electrophoresis

目的：建立膝沟藻毒素（gonyautoxin，GTX）2和3的毛细管电泳分析方法。用毛细管电泳法分析和定量GTX免疫原。方法：用磷酸氢二钠-柠檬酸为运行缓冲液的区带毛细管电泳紫外检测法，对GTX2和3进行检测。结果：GTX2在0．5—118．0μmol／L，GTX3在0．5—39．0μmol／L时，有良好的线性关系。GTX2最低检测限为0．38μmol／L，GTX3最低检测限为0．36μmol／L（S／N≥3）。日内精密度为：GTX2是2．5％，GTX3是2．6％；日间精密度是：GTX2为5．6％，GTX3为6．3％。用该法定量GTX2＆3免疫原中GTX2的浓度为325．8±32．8μmol／L，GTX3的浓度为287．6±15．1μmol／L。结论：用磷酸氢二钠-柠檬酸为运行缓冲液的高效毛细管电泳法，可对GTX2＆3相关样品进行快速而又灵敏的分析和定量。

Objectives：To establish a method to determine gonyautoxin （GTX） 2＆3 by capillary electrophoresis, Methods： This assay was based on the ultraviolet absorbance of GTX 2＆3 at zone capillary electrophoresis. The running buffer was disodium hydrogen phosphate - citric acid. Results：The linearity of GTX2 was from 0, 5 μmol/L to 118.0 μmol/L, and GTX3 from 0. 5 μmol/L to 39. 0 μmol/L. The minimal detection limit of GTX2 was 0. 38 μmol/L, and GTX3 was 0. 36 μmol/L（S/N≥3）. With- in - run precision of GTX2 was 2. 5%, and GTX3 2. 6%, Between - run precision of GTX2 was 5. 6% ,and GTX3 6. 3%. Concentration of immunogen GTX2 was quantified as 325.8 ± 32, 8 μmol/L, and GTX3 quantified as 287.6 ± 15.1 μmol/L. Conclusion：GTX 2＆3 can be quantified rapidly and sensitively by high performance capillary electrophoresis in disodium hydrogen phosphate - citric acid buffer system.