HPLC-PDA法检验保健食品中的盐酸西布曲明

Determination of sibutramine hydrochloride in health food by HPLC-PDA

目的：以紫外检测器检测，根据色谱峰的绝对保留时间定性，对可疑阳性样品二极管阵列检测器再定性，用外标法峰面积定量，建立保健食品中盐酸西布曲明的测定方法。方法：采用反相高效液相色谱法，Symmetry C18（4．6×250mm，5μm）色谱柱，流动相为甲醇-水-三乙胺（75：25：0．1），流速为1．0ml／min，检测波长为223nm.结果：其工作曲线的线性范围：10—200μg／ml，相关系数：r=0．9999，回收率：97．07％，RSD：1．39％。结论：方法准确、可靠，可用于保健品中盐酸西布曲明的检测。

Objective： To establish an HPLC method for the determination of sibutramine hydrochloride in health food. Ultraviolet detector and photodiode array detector were used based on chromatogram apex＇s absolute reserve time and photodiode array spectrum for qualitative analysis and external standard method with peak area for quantitative analysis. Methods： Reversed phase high performance liquid chromatography was used. The column was symmetry C18 （4.6 × 250 mm,5 μm）, the mobile phase was methanol ： water： triehylamine = 75 ： 25：0. 1 （ v： v： v） with flow rate 1.0 ml/min and the detective wavelength was 223 nm. Results：The linear range of the calibration curve of sibutramine hydrochloride was 10 -200 μg/ml （ r =0. 9999）. The rate of recovery was 97.07 %, RSD was 1.39%. Conclusion：The method is accurate, realiable that can be used to determine sibutramine hydrochloride in health food.