摘要
目的:研究慢性肝病血清中可溶性I型补体受体(sCR1)的浓度变化和层粘连蛋白的含量变化,并分析二者的相关性,以探讨其临床意义。方法:采用双抗体夹心ELISA法测定血清中sCR1浓度,采用放射免疫法测定血清层粘连蛋白含量。结果:对慢性肝病人群及正常人群进行研究表明,不同肝病患者血清sCR1水平不同程度的升高,慢性肝病血清sCR1浓度显著高于正常人群(P<0.01);并且23例肝硬化患者血清sCR1浓度显著高于慢性肝病人群(P<0.01);血清sCR1与层粘连蛋白(LN)二者密切相关(r=0.73)。结论:慢性肝病血清sCR1的变化、层粘连蛋白含量的变化与肝脏损伤严重程度密切相关,可为慢性肝病患者的早期、快速、准确诊断提供相应的理论依据。
Objective:To study the changes of concentrations of soluble complement receptor type 1 ( sCR1 ) and laminin in serum of patients with chronic liver diseases, analyze their relationship and investigate the significance of sCR1 and laminin. Methods: Enzyme- linked immunosorbent assay (ELISA) was used in detecting the concentration of sCR1 in serum, and radioimmuassay was used in detecting the laminin in serum. Results :The results showed that the concentration of serum sCR1 in liver diseaseswas significantly higher than that of healthy individuals ( P 〈 0. 01 ). The serum sCR1 of 23 hepatocirrhosis was significantly higher than that of patients with chronic liver diseases ( P 〈 0. 01 ), and correlation with laminin( r = 0.73 ). Conclusion: sCR1 accumulates in liver diseases and evaluation of its serum levels could be useful as a liver function test. It could act as a proof in early, rapid and correct diagnosis of chronic liver diseases.
出处
《中国卫生检验杂志》
CAS
2007年第3期481-482,489,共3页
Chinese Journal of Health Laboratory Technology