HPLC同时测定食品中黄曲霉毒素B1、B2、G1、G2

Simultaneous detection of aflatoxin B1,B2,G1,G2 in foods by HPLC

目的:分析常见种类食物中黄曲霉毒素的含量,为建立食品中黄曲霉毒素的限量指标提供数据,并为大众的健康饮食提供参考。方法:以黄曲霉毒素B1的平均含量为参考选择黄曲霉毒素含量较多的常见食物种类,使用高效液相色谱仪及荧光检测器分析食物中黄曲霉毒素B1、B2、G1、G2的含量。结果:花生酱中含有的黄曲霉毒素最多,平均为2.41μg/kg;大米中黄曲霉毒素的含量次之,平均为0.15μg/kg;花生中黄曲霉毒素的平均含量为0.04μg/kg。结论:高效液相色谱法测定食物中黄曲霉毒素其重现性较好,而且可以一次分别测定出黄曲霉毒素B1、B2、G1、G2的含量,从结果看来尽管多数食品中均含有黄曲霉毒素,但是其含量均没有超过国家限量标准,其中花生酱中的黄曲霉毒素含量较高。这些数据均可以为大众的健康饮食提供参考。

Objective：To determine the aflatoxin （AF B1 ,B2,G1 ,G2） in different kinds of food so as to provide the refer- ences for guiding the diet for people. Methods：Those food samples whose contents of AF B1, B2, G1, G2 were more than others were determined by HPLC. Results：The average AF in peanut butter was 2. 41μg/kg. The average AF in rice was 0. 15 μg/kg, and the average AF in peanuts was 0. 04 μg/kg. Conclusion：The repeatability of the analysis method is good. The method can be used to determine the content of AF B1, AF B2, AF G1 ,AF G2 respectively once time and these data can guide the diet of people.