草地早熟禾愈伤组织遗传转化受体系统的建立

Establishment of the Transformation Receptor System for Poa pratensis

以草地早熟禾成熟胚为外植体,并去除部分胚乳,或用浓盐酸处理,选择NB+2,4-D 2mg/L为诱导培养基,NB+6-BA 2mg/L为分化培养基,1/2NB+IAA 0.5mg/L+NAA 0.5mg/L为生根培养基,建立了这些品种的胚性愈伤组织高频诱导与再生系统,确定了G418(40-50mg/L)Hpt(50mg/L)的筛选临界浓度,确定了最佳分化时间,探讨了建立草地早熟禾转化受体系统的必要条件。

An efficient regeneration and transformation system for Poa pratensis L. was studied by using mature seeds of three species. An efficient induction and regeneration system from embryonic calli of these species was established. Mature seeds was used as the explant, wiped off part of endosperm and treated with concentrated hydrochloric acid; choose NB＋ 2,4-- D 2mg/L ag induction culture medium, NB＋ 6 -- BA 2mg/L as differential medium, 1/2NB＋IAA 0.5mg/L＋NAA 0. 5mg/L as radication medium） confirmed the concentrations of different antibiotics for callus＇ growth： G418 （40 - 50mg/L） and Hpt （50 mg/L）. The essensial requirement of establishing the transformation receptor system for P. pratensis L. was discussed from choosing plant tissues, confirming the effect medias and selecting the best regeneration time.