桔梗药材中桔梗皂苷D的定性定量方法研究

Study on qualitative and quantitative methods of platycodin D in roots of Platycodon grandiflorus

目的进行桔梗药材的质量标准研究。方法以桔梗皂苷D为对照品，采用TLC法进行定性鉴别，HPLC法进行含量测定，Zorbax C18柱（4．6mm×150mm，5um），流动相为甲醇-乙腈-0．2％磷酸水溶液（3：25：72），检测波长为204nm，柱温为40℃，流速为1．0mL/min。结果桔梗皂苷D在0．226-2．486ug线性关系良好（r=1．0000），平均回收率101．81％，RSD1．70％。结论通过10批次不同产地桔梗的鉴别和含量测定，证明本方法灵敏，准确可靠，重现性好，可用于桔梗药材的质量控制。

Objective To establish a quality standard of Platycodon grandiflorus. Methods The presence of platycodin D were identified and assayed by TLC and HPLC, respectively. Extracts were separated on a Zorbax C18 column with methanol-acetonitrile-0.2% phosphoric acid （3： 25： 72） as mobile phase. The detection wavelength was set at 204 nm. The column temperature was 40 ℃ and flow-rate was 1.0 mL/min. Results The linearity was obtained in the range of 0. 226 -2. 486 ug（ r = 1. 000 0）. The average recovery rate was 101.81% （ RSD = 1.70% ）. Conclusion The methods were sensitive, accurate, reliable, and suitable for the quality control of Platycodon grandiflorus.