G-CSF治疗大鼠急性心肌梗死的实验研究

Effects of G-CSF in Treatment of Acute Myocardial Infarction in Rats

目的:观察应用G-CSF(粒细胞集落刺激因子)对大鼠AMI(急性心肌梗死)的治疗作用。方法:将32只雄性SD大鼠用异丙基肾上腺素(ISO)复制AMI模型,3 h后随机分为两组(每组16只)。A组:G-CSF组;B组:对照组。各组随机分为2个亚组,分别于2周和4周测定心室功能,同步记录心率;然后处死,取出心脏,称重;HE染色,体视学方法检测心梗面积。结果:(1)给药后14 d:1心室功能:A组左心室收缩压(LVSP)、左心室舒张末压(LVEDP)、左心室内压最大上升/下降速度(±dp/dt)均大于B组(P<0.05);2左心室重量:A组心脏重/体重(g/G)小于B组(P<0.05);3心肌梗死面积:A组心梗面积/左心室总面积(s/S)小于B组。(2)给药后28 d1心室功能:A组LVSP、LVEDP、±dp/dt均大于B组(P<0.05);2左心室重量:A组g/G小于B组(P<0.05);3心肌梗死面积:A组s/S小于B组(P<0.05);(3)组内比较:1A组4周亚组较2周亚组LVSP、LVEDP、±dp/dt提高(P<0.05),心梗面积减小(P<0.05);心室重塑不明显。2B组4周亚组较2周亚组各指标差异无显著性。以上各组间及组内HR统计差异无显著性。结论:G-CSF对AMI大鼠缺血损伤心肌有保护和再生作用,可明显改善AMI大鼠的心室功能。

Objective：To investigate the effects of granulocyte colony-stimulating factor（G-CSF） mobilizing bone stem cells in treatment of acute myocardial infarction （AMI） in rats. Methods.. 32 SD masculine rats were randomly divided into 2 groups （every group possess 16 rats） after injected isoproterenol （ISO） and created AMI model. A group：G- CSF group ,B group ：control group. Every group was randomly divided into 2 subgroups. Hearts of every group was measured ventricle function and simultaneity weighted the heart after 2 weeks and 4 weeks. HE stain was used to detect the myocardial infarction areas. Results： （1）14 days after ISO （1）the left ventricle function, in group A, the LVSP, LVEDP, ± dp/dt were higher than B group （P〈0.05） ,（2）the weight of left ventricle：in group A, the weight of heart/avoirdupois of rat was less than B group （P〈0.05）;（3）the myocardial infarction areas ：in group A,the myocardial infarction areas/the left ventricle overall areas was less than B group （P〈0.05）;（2）In group A and 13,there were the same results for 28 days comparing with for 14 days after injecting ISO. （3）the results of comparing between subgroups （1）In A group,the ventricle function in the 4 weeks subgroup was higher than in the 2 weeks subgroup （P〈0.05）,the myocardial infarction areas in the 4 weeks subgroup were smaller than in the 2 weeks subgroup （P〈0.05）. The remodification of ventricle was not significant. （2）In B group,there were not significance difference of ventricle function,myocardial infarction areas and remodification of the left ventricle between subgroups. There was not statistics significance difference of heart rate between groups. Conclusions：G-CSF can protect and rebirth the ischemiced cardiac muscle of acute myocardial infarction in the rat models and improve the ventricle function in rats.