农杆菌介导的β-1,3-葡聚糖酶基因转化花生的研究

Studies on Transformation of Peanut with BG_2 Gene Mediated by Agrobacterium tumefaciens

以花育22号成熟胚萌发5-7 d的幼叶为外植体,以农杆菌为介导将含有β-1,3-葡聚糖酶基因的表达载体pATC940转入花生,获得转基因植株。结果表明,外植体经预培养1-2 d,于OD600为0.5-1.0之间的农杆菌菌液中浸泡10-15 min,再共培养3 d,有利于提高转化率。并对再生的抗性植株进行了PCR检测。

The genetic transformation of peanut young leaflets was studied, Plant expression vector pATC940 harboring β-1,3-glucanase （BGz） gene was transferred into peanut mediated by Agrobacterium tumefaciens. The optimized procedure was as following. After pre-cultured for 1-2 days, the young leaflets were dipped into an Agrobacterium suspension （OD600=0.5-1.0） for 10- 15 min, Then the leaflets were co-cultured with Agrobacterium tumefaciens strain LBA 4404. Three days later, the explants were transferred to shoot induction medium, These conditions benefited to improve the transformation efficiency, PCR analysis showed that transgenic peanut plants were obtained,