HPLC法测定乙美片中乙水杨胺、咖啡因和美索巴莫的含量

HPLC determination of ethenzamide,caffeine and methocarbamol in compound methocarbamol tablets

目的:建立乙美片中乙水杨胺、咖啡因和美索巴莫的含量测定方法。方法:采用 HPLC 法测定,C_(18)柱(150 mm×4.6 mm,5μm),以甲醇-水-冰醋酸(39:61:1)为流动相,流速为1 mL·min^(-1),紫外检测波长为280 nm,柱温:室温;进样量:20μL。结果:乙水杨胺、咖啡因和美索巴莫线性范围分别为0.16～1.48μg,0.0163～0.1465μg,0.275～2.475μg;平均回收率(n=9)分别为99.5%(RSD 为0.78%),99.7%(RSD 为0.95%),100.6%(RSD 为0.98%)。结论:本方法简便、准确,可为评价乙美片中乙水杨胺、咖啡因和美索巴莫的含量提供依据。

Objective： To establish a method for the determination of ethenzamide, caffeine and methocarbamol in compound methocarbamol tablets. Methods：The HPLC column was C18 （150 mm ×4. 6 mm,5 μm）;the mobile phase was methanol - water - acetic acid （39：61 ： 1 ） ; the flow rate was 1 mL · min^ - 1, the UV detection wavelength was 280 nm, room temperature for column. Loaded volume was 20 μL. Results：The calibration curves of ethenzamide, caffeine and methocarbamol were linear in the range of 0. 16 - 1.48 μg,0. 0163 - 0. 1465 μg,0. 275 - 2. 475 μg respectively ; The average recoveries （ n = 9 ） were 99.5% （ RSD 0. 78% ） , 99. 7% （ RSD 0. 95% ） and 100. 6% （RSD 0. 98% ）respectively. Conclusion： The method is rapid , accurate and reliable.