摘要
目的:建立流动注射-褪色光度法测定药物中维生素 C 含量的方法。方法:盐酸介质中,维生素 C(Vc)能使 Fe^(3+)与SCN^-红色络合物褪色,且络合物吸光度减小与 Vc 用量成线性关系,流动注射体系测定褪色前后络合物吸光度。测定波长:480 nm,流速:6.7 mL·min^(-1)。结果:在优化条件下,Vc 浓度在0.0~6.4μg·mL^(-1)范围内,符合朗伯-比耳定律,线性方程为△A=-0.00406+0.08347C,r=0.9995,检出限(S/N=3)为0.18μg·mL^(-1),流通量为72样/h。使用本法测定了 Vc 片剂、注射液及银翘片中的 Vc,结果与药典法一致。结论:本法快速准确,可用于 Vc 的批量分析。
Objective: To establish a method for determination of vitamin C by FIA - discoloring spectrophotometry. Method : In the medium of HCl, a new FIA - discoloring spectrophotometry for the determination of Vc was proposed based on the discoloring effect of Vc on the reaction between Fe^3 + and SCN - and the absorbance drop of red complex of Fe^3 + and SCN - was proportional to the amount of Vc. The detection wavelength was 480 nm and flow rate was 6.7 mL· min^ - 1. Results. Under the optimum conditions, the linear range and detection limit was 0. 0 - 6.4 μg · mL^ - 1 ( r = 0. 9995 ) and 0. 18 μg · mL^ - 1 respectively. The sample throughput was 72 samples/h. The proposed method has been successfully applied to the determination of Vc in the Vc tablets, Vc injection and cimetidine tablets. Conclusion:The method is simple, accurate and very suitable to determine Vc in batches.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2007年第3期450-453,共4页
Chinese Journal of Pharmaceutical Analysis