生物功能催化膜定点固定化技术研究进展

Research Process of Site-specifically Immobilization in Catalytic Biofunctional Membranes

介绍了从生物物理学、生物分析化学、化学工程学和分子生物学等学科角度研究开发的基于定点固定化技术的生物功能催化膜。着重阐述了基因定点突变技术、基因融合技术和翻译修饰技术等新兴定点固定化技术的原理、特点和操作。最后,对生物功能催化膜的研究方向进行了展望。

The efforts of developing catalytic biofunetional membranes eantaining site- specifically immobilized enzyme was summarized involving biophysical chemistry and bioanalytieal chemistry and chemical engineering, together with molecular biology. Several kinds of novel immobilized technology were employed to effectuate the site - specific enzyme membrane immobilization. The principles, operation and applications of site - directed mutagenesis, gene fusion technology, and post- translational modification methods were introduced emphatically, Finally, promising research directions of catalytic biofunefional membranes were predicted.