中国7个毛木耳(Auricularia polytricha)菌株ITS序列比较

ITS Sequence Comparison of Germplasm Resources of Auricularia polytricha

对毛木耳7个菌株rRNA基因内转录间区(ITS)进行了克隆测序,并将其与木耳属其它几种的相应序列进行了比较.在供试的7个毛木耳菌株中,除特大(209bp)外,其余6个菌株的ITS2区序列长度完全相同;毛木耳rRNA基因的两个ITS区序列有一定数量的碱基变异,整个ITS区共有11个变异位点.与下载的木耳属另外4个种相比较,均有较大程度的变异.根据遗传同源性分析,遗传距离分析和系统树上所显示的供试菌株及相关已知种的亲缘关系,ITS序列分析支持传统的依据形态学进行的木耳属分类.

The internal transcribed spacer （ITS） region in rRNA gene of Auricularia polytricha was cloned and sequenced, and the sequences were compared with those of some common species of Auricularia. The results showed that 6 strains among tested strains, except Teda with different origins, had the same length of ITS2. There were base variances in ITS region of rRNA gene among the tested strains, and with 11 variance loci in ITS region. Compared with other 4 species from the GenBank, ITS1 and ITS2 sequences of A. polytricha varied in great extent. According to the analysis of distance matrix, the genetic relationship between strains and the related known species from systematic dendrogram, ITS sequence analysis supports the traditional classification of Auricularia based on morphology. Fig 2, Tab 2, Ref 9