大肠杆菌对赤子爱胜蚓体表超微结构的影响

Effect of Escherichia coli on Ultrastructure Integumentary System of Eisenia fetida

以赤子爱胜蚓(Eisenia fetida)为实验动物,研究了细菌诱导(浸浴诱导和注射诱导)对蚯蚓体表超微结构的影响.结果表明:(1)用细菌浸浴4h的实验组、用细菌注射4h的实验组及对照组蚯蚓的角质层厚度分别为(0.51±0.03)μm、(0.56±0.02)μm、(0.50±0.01)μm;上角质层突起的高度分别为(0.16±0.02)μm、(0.12±0.01)μm、(0.11±0.01)μm;上角质层游离面中微绒毛的高度分别为(0.18±0.02)μm、(0.11±0.01)μm、(0.11±0.01)μm.统计分析表明,蚯蚓在抵御微生物侵袭过程中,体表角质层中的上角质层突起及上角质层游离面中的微绒毛发挥着重要作用.(2)细菌浸浴4h的实验组、用细菌注射4h实验组及对照组蚯蚓体表的表皮厚度分别为(2.63±0.15)μm、(2.23±0.06)μm、(1.97±0.15)μm;表皮中小颗粒蛋白细胞的颗粒直径分别为(0.38±0.04)μm、(0.26±0.02)μm、(0.24±0.02)μm;大颗粒粘液细胞的颗粒直径分别为(0.50±0.04)μm、(0.26±0.03)μm、(0.21±0.02)μm;网状粘液细胞的直径分别为(0.93±0.06)μm、(0.63±0.06)μm、(0.28±0.03)μm.统计分析表明,蚯蚓在抵御微生物侵袭的过程中,表皮中的腺细胞(小颗粒蛋白细胞、大颗粒粘液细胞及网状粘液细胞)发挥着重要作用.

The effect of bacterial inducement on the ultrastructure integumentary system of earthworm （Eisenia fetida） was studied. The results showed that ： （ 1 ） Of the 4-hour immersed group of Escherichia coli, the 4-hour injected group of E. coli and the control group, the cuticles of E. fetida were （0.51 0.03） μm, （0.56 ±0.02） μm and （0.50 ±0.01） μm; the upcuticle protuberances of E. fetida were （0.16 ±0.02） μm, （0.12 -±0.01 ） μm, and （0.11 ±0.01 ） μm; the micro-flocci in the up-cuticle dissociation surface ofE. fetida were （0.18 ±0.02） μm, （0.11 ±0.01） μm and （0.11 ±0.01） μm, respectively. According with the statistics results, it could be found that the up-cuticle protuberances and the micro-flocci in the up-cuticle dissociation surface of E. fetida cuticle might have the important function in the course of resisting the invasion of E. coli. （2） Of the 4-hour immersed group of E. coli, the 4-hour injected group of E. coli and the control group, the epidermis of E. fetida were （2.63 ±0.15） μm, （2.23±0.06） μm and （1.97±0.15） μm; the diameters of small proteinaceous granule in the epidermis of E. fetida were （0.38 ± 0.04） μm, （0.26 ± 0.02） μm and （0.24 ± 0.02） μm ; the diameters of large mucous granule in the epidermis of E. fetida were （0.50 ± 0.04） μm, （0.26 ± 0.03） μm and （0.21 ± 0.02） μm; the diameters of reticulation mucous cells in the epidermis of E. fetida were （0.93 ± 0.06） μm, （0.63 ± 0.06） μm and （0.28 ± 0.03） μm, respectively. According to the statistics results, it could be found that the gland cells in the epidermis of E. fetida, such as small granular proteinaceous cells, large granular orthochromatic mucous cells and reticulation mucous cells might have the important function in the course of resisting the invasion of E. coli. Fig 4, Tab 2, R.ef 17